The generation of high quality plasma without the use of a centrifuge is becoming a focus of point-of-care diagnostic assays. Many assays cannot accept the inherent variation of whole blood and require the generation of plasma. When selecting plasma separation membranes, you must consider the following factors:
Blood Volume – For optimal separation, you must understand the volume limitations of the membrane so that it is not overloaded. Not all plasma separation materials can accept the same amount of whole blood.
Plasma Volume – The amount of plasma generated from filtration through a membrane variesdepending on the polymer type. The two most commonly used substrates for plasma generation are asymmetric polysulfone and glass fiber. Glass fiber materials typically generate less plasma per microliter of blood than other materials.
Hemolysis – It is well known that glass fiber materials can hemolyze red cells which can impact the downstream analysis. It is important to understand the amount of hemolysis your assay can handle as you approach the selection of a plasma separation material .
Separation Time – If your assay has time constraints, you should understand how quickly the material separates plasma. Separation times will vary depending on the material selected.
Non-specific Binding – Although glass fiber materials are commonly used in blood separation applications, they present certain performance limitations. It is common for these materials to bind target analytes such as lipids (e.g., cholesterol). It is imperative that the binding characteristics of the blood separation material are evaluated to ensure that the biomarker of interest is not bound by the filter matrix.
Our patented Vivid™ Plasma Separation membranes are robust materials for the rapid and efficient separation of plasma from whole blood. The material separates > 80% of the theoretical plasma available from the whole blood sample with minimal hemolysis in less than two minutes. Pall has multiple versions available depending on the blood volume requirements of your assay.
Vivid™ Plasma Separation Membrane